Sheer velocity dating in the dark
Direct delivery of nucleic acids as described in this work offers a compelling alternative that avoids the inherent shortcomings of viral vectors.
The shear mechanoporation method first reported by Zarnitsyn et al.
Physical (non-viral, non-chemical) approaches to delivery include direct insertion and field-mediated disruption of the cell membrane (electrical, mechanical/acoustic, shear, optical or thermal).
There is a major potential for extension of CAR-T cell therapy to other hematologic malignancies (e.g., multiple myeloma) and many solid tumors; however, existing approved CAR-T cell therapies and those under development all use effective yet undesirable viral vectors for nucleic acid delivery.Historically, significant effort has focused on strategies for effective DNA and RNA delivery; however, the predominant methods for in vivo (viral) and in vitro (liposomal) transfection are not well-suited to delivery of proteins, small molecules, quantum dots and other nanoparticles of interest in emerging clinical and laboratory applications (e.g., cell reprogramming).Many small lipophilic molecules spontaneously cross biological membranes.In practice, this unique capability is negated by the low throughput of the method.Field-mediated membrane poration has supplanted chemical methods in many delivery applications, particularly those involving nongene target molecules and primary cells.